Within the network of Paris-Saclay proteomics platforms (P2S), and affiliated to the Graduate School of Health and Drug Science (GS HeaDS), PROTEOMIC - IPSIT facility is open to researchers dedicated to pharmaceutical sciences belonging to academic or private laboratories.
PROTEOMIC - IPSIT collaborates with users by providing them technologies and expertise for qualitative and quantitative proteome analysis.

PROTEOMIC - IPSIT is listed in Pluginlabs

PRESTATIONS

In the context of the proteome study, Bottom-Up strategy is applied. This approach involves first digesting proteins and then analyzing peptides mixture by LCMS.
The inherent complexity of the peptides mixture is resolved through nano liquid chromatography-tandem mass spectrometry analysis (LCMS). Peptides are separated under a gradient of acetonitrile on a reverse phase column. Reaching the High Resolution Mass Spectrometer, peptides are ionized by positive electrospray (ESI) and monitored depending on their mass/charge ratio (m/z). Under gas-phase collision-activated dissociation (CAD) peptides backbones are cleaved at peptide amide bonds to produce specific pattern of fragments (MS/MS).
Post-translational modifications (PTMs) can be studied, depending on their nature and stability under analysis conditions in place.
The system also allows analysis of purified intact proteins under certain conditions.

Following a meeting between the facility members and the project leader(s) to validate the feasibility of the experiments, the analyses are carried out according to the proteomics collaboration request form, filled in by mutual agreement and under conditions of collaboration described in the charter of the PROTEOMIC - IPSIT facility.

EQUIPEMENTS

Xevo G2-XS QTof - ACQUITY UPLC M-Class system (WATERS) :
Funding : Région Ile-de-France (convention EX024607), Université Paris-Saclay, INSERM and CNRS :